Gene-expression profiling of peripheral blood mononuclear cells in sepsis - GSE9960 [Sepsis, SIRS]
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| Description | Gene-expression profiling of peripheral blood mononuclear cells in sepsis - GSE9960 |
| Purpose | To identify signature genes that help distinguish (1) sepsis from non-infectious causes of systemic inflammatory response syndrome, (2) between Gram-positive and Gram-negative sepsis. |
| Experimental Design | A total of 70 critically ill patients were enrolled in a single-centre observational study. Gene-expression profiling was performed using Affymetrix microarray (U133plus2) with 54,675 transcript. Data was divided into a training set (n=35) and a validation set (n=35). A molecular signature was developed in the training set and was then validated in the validation set. |
| Experimental Variables | Sepsis (2021 ICD-10-CM code* = A41) |
| Methods | Affymetrix CEL files were imported into the RMA software (Bioconductor) to compute probeset summaries. Quantile nomalization was used. |
| Additional Information | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE9960 |
| Platform | Affymetrix HG-U133_Plus_2 |
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