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Clinical

Whole blood mRNA expression profiling of host molecular networks in neonatal sepsis. Subset of GSE25504 using platform GPL570

Purpose

We aimed to identify the gene network and pathway biology associated with neonatal sepsis by determining genome-wide alterations in host RNA in infected infants

Hypothesis

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Experimental Design

Samples were obtained from control and infected human neonates.

Experimental Variables

Sepsis (2021 ICD-10-CM code* = A41)

Controls

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Methods

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Additional Information

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Microarray
Affymetrix HG-U133_Plus_2
5 Samples Loaded: 5
Human (Homo sapiens)
Whole Blood
Sepsis
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample Title Gender tissue Grouping Birthweight (g)
GSM1404208 Con006 Male peripheral blood Control 4240
GSM1404209 Con014 Female peripheral blood Control 2970
GSM1404210 Con030 Male peripheral blood Control 3270
GSM1404211 Inf012 Female peripheral blood Infected 2150
GSM1404212 Inf032 Female peripheral blood Infected 3180
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File
GSE25504 GPL570 modified.csv
Sample Set Spreadsheet
sampleset4000105_sampleannotationsGSE25504_GPL570_new.csv
Sample Set Spreadsheet
Log2 Transformed
Raw Signal
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Sample ID !Sample Title Gender Tissue Grouping Birthweight (g) Bloodculture result Corrected gestational age (weeks+days) On antibiotics at time of sample Other positive results
GSM1404208
Con006
Male
peripheral blood
Control
4240
negative
41+3
No
no
GSM1404209
Con014
Female
peripheral blood
Control
2970
na
38+3
No
no
GSM1404210
Con030
Male
peripheral blood
Control
3270
na
40+1
No
umbilical swab grew proteus (may represent colonisation)
GSM1404211
Inf012
Female
peripheral blood
Infected
2150
negative
34+6
No
CSF: enterovirus on PCR. CSF also grew coag neg staph, CSF WCC 300, RBC 9
GSM1404212
Inf032
Female
peripheral blood
Infected
3180
negative
41+1
No
yes CSF grew Listeria monocytogenes, CSF white cell count 551, RBC 119, urine no growth

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