Sample Set Annotation Tool: Stimulation by LPS and HMGB1 in peripheral blood neutrophils from patients with sepsis-induced acute lung injury - GSE3037 [Sepsis, Lipopolysaccharide (LPS) and HMGB1 exposure]

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Peripheral blood neutrophils were isolated from septic patients and treated in vitro with LPS or HMGB1

Hypothesis

Experimental Design

8 patients, each contributing a control, LPS-treated, and HMGB1-treated neutrophil sample.

Experimental Variables

Lipopolysaccharide and HMGB1 exposure (2021 ICD-10-CM code* = Not Applicable)

Controls

Methods

GCRMA

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE3037

Expected Samples:

24 Samples Loaded: 24

Species

Human (Homo sapiens)

Sample Source

Neutrophils

Disease

Lipopolysaccharide and HMGB1 exposure

Treatment Protocol

Growth Protocol

Extraction Protocol

Approximately 20 million peripheral blood neutrophils, previously stabilized in RNAlater (Ambion), were lysed in Trizol (Life Technologies) and total RNA isolated with Rneasy MinElute columns (Qiagen).

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	Grouping	None provided
GSM66867	Patient 05 control	Control	TRUE
GSM66868	Patient 06 control	Control	TRUE
GSM66869	Patient 07 control	Control	TRUE
GSM66870	Patient 08 control	Control	TRUE
GSM66871	Patient 09 control	Control	TRUE

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File
sampleset4000124_sampleannotations.csv Sample Set Spreadsheet
sampleset4000124_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Grouping	None provided
GSM66867	Patient 05 control	Control	TRUE
GSM66868	Patient 06 control	Control	TRUE
GSM66869	Patient 07 control	Control	TRUE
GSM66870	Patient 08 control	Control	TRUE
GSM66871	Patient 09 control	Control	TRUE
GSM66872	Patient 12 control	Control	TRUE
GSM66873	Patient 15 control	Control	TRUE
GSM66874	Patient 19 control	Control	TRUE
GSM66875	Patient 05 HMGB1 treated	HMGB1	TRUE
GSM66876	Patient 06 HMGB1 treated	HMGB1	TRUE
GSM66877	Patient 07 HMGB1 treated	HMGB1	TRUE
GSM66878	Patient 08 HMGB1 treated	HMGB1	TRUE
GSM66879	Patient 09 HMGB1 treated	HMGB1	TRUE
GSM66880	Patient 12 HMGB1 treated	HMGB1	TRUE
GSM66881	Patient 15 HMGB1 treated	HMGB1	TRUE
GSM66882	Patient 19 HMGB1 treated	HMGB1	TRUE
GSM66883	Patient 05 LPS treated	LPS	TRUE
GSM66884	Patient 06 LPS treated	LPS	TRUE
GSM66885	Patient 07 LPS treated	LPS	TRUE
GSM66886	Patient 08 LPS treated	LPS	TRUE
GSM66887	Patient 09 LPS treated	LPS	TRUE
GSM66888	Patient 12 LPS treated	LPS	TRUE
GSM66889	Patient 15 LPS treated	LPS	TRUE
GSM66890	Patient 19 LPS treated	LPS	TRUE

Group Sets View in Gene Expression Browser

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SIDRA