Sample Set Annotation Tool: Transcriptome of the septic shock after treatment by activated protein C (Xigris)

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Septic shock by pneumopathy was studied in prospective way in 20 patients before and after respective treatment by activated protein C (Xigris). Initial blood samples were taken within 12 hours of diagnosis and the resulting transcritomes were compared to samples drawn after respective treatment.keywords: patient cohort study, septic shock, transcription-profile, blood

Hypothesis

Experimental Design

In total 20 patients were tested before and after specific treatment.

Experimental Variables

Activated protein C treatment for septic shock (2021 ICD-10-CM code* = R65.21)

Controls

Methods

RMA; quality control was performed using RReportGenerator (Raffelsberger et al, 2008, PMID:18037684)

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE33119

Expected Samples:

40 Samples Loaded: 40

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

Activated protein C treatment for septic shock

Treatment Protocol

Growth Protocol

Extraction Protocol

Blood samples were taken from patients. Total RNA used for microarray studies was isolated using a PAXgene Blood RNA kit (Quiagen, Valencia, CA).

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	Age	!Sample_title	infection	tissue	Sex
GSM830309	60-79	blood_patient01_t0	pneumonia	blood	M
GSM830310	60-79	blood_patient01_t1	pneumonia	blood	M
GSM830311	40-59	blood_patient02_t0	pneumonia	blood	M
GSM830312	40-59	blood_patient02_t1	pneumonia	blood	M
GSM830313	40-59	blood_patient03_t0	pneumonia	blood	M

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File

Samples Viewer / Editor

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Sample ID	Age	!Sample Title	Infection	Tissue	Sex	Treatment	Survival	Time-point
GSM830309	60-79	blood_patient01_t0	pneumonia	blood	M	none	negative	early
GSM830310	60-79	blood_patient01_t1	pneumonia	blood	M	activated protein C	negative	late
GSM830311	40-59	blood_patient02_t0	pneumonia	blood	M	none	negative	early
GSM830312	40-59	blood_patient02_t1	pneumonia	blood	M	activated protein C	negative	late
GSM830313	40-59	blood_patient03_t0	pneumonia	blood	M	none	positive	early
GSM830314	40-59	blood_patient03_t1	pneumonia	blood	M	activated protein C	positive	late
GSM830315	60-79	blood_patient04_t0	pneumonia	blood	F	none	positive	early
GSM830316	60-79	blood_patient04_t1	pneumonia	blood	F	activated protein C	positive	late
GSM830317	40-59	blood_patient05_t0	pneumonia	blood	F	none	positive	early
GSM830318	40-59	blood_patient05_t1	pneumonia	blood	F	activated protein C	positive	late
GSM830319	20-39	blood_patient06_t0	pneumonia	blood	F	none	negative	early
GSM830320	20-39	blood_patient06_t1	pneumonia	blood	F	activated protein C	negative	late
GSM830321	60-79	blood_patient07_t0	pneumonia	blood	F	none	negative	early
GSM830322	60-79	blood_patient07_t1	pneumonia	blood	F	activated protein C	negative	late
GSM830323	> 80	blood_patient08_t0	pneumonia	blood	F	none	positive	early
GSM830324	> 80	blood_patient08_t1	pneumonia	blood	F	activated protein C	positive	late
GSM830325	> 80	blood_patient09_t0	pneumonia	blood	M	none	positive	early
GSM830326	> 80	blood_patient09_t1	pneumonia	blood	M	activated protein C	positive	late
GSM830327	> 80	blood_patient10_t0	pneumonia	blood	F	none	negative	early
GSM830328	> 80	blood_patient10_t1	pneumonia	blood	F	activated protein C	negative	late
GSM830329	60-79	blood_patient11_t0	pneumonia	blood	F	none	positive	early
GSM830330	60-79	blood_patient11_t1	pneumonia	blood	F	none	positive	late
GSM830331	60-79	blood_patient12_t0	pneumonia	blood	F	none	negative	early
GSM830332	60-79	blood_patient12_t1	pneumonia	blood	F	none	negative	late
GSM830333	60-79	blood_patient13_t0	pneumonia	blood	M	none	negative	early
GSM830334	60-79	blood_patient13_t1	pneumonia	blood	M	none	negative	late
GSM830335	60-79	blood_patient14_t0	pneumonia	blood	M	none	negative	early
GSM830336	60-79	blood_patient14_t1	pneumonia	blood	M	none	negative	late
GSM830337	20-39	blood_patient15_t0	pneumonia	blood	M	none	positive	early
GSM830338	20-39	blood_patient15_t1	pneumonia	blood	M	none	positive	late
GSM830339	60-79	blood_patient16_t0	pneumonia	blood	M	none	negative	early
GSM830340	60-79	blood_patient16_t1	pneumonia	blood	M	none	negative	late
GSM830341	> 80	blood_patient17_t0	pneumonia	blood	M	none	negative	early
GSM830342	> 80	blood_patient17_t1	pneumonia	blood	M	none	negative	late
GSM830343	> 80	blood_patient18_t0	pneumonia	blood	M	none	positive	early
GSM830344	> 80	blood_patient18_t1	pneumonia	blood	M	none	positive	late
GSM830345	60-79	blood_patient19_t0	pneumonia	blood	M	none	positive	early
GSM830346	60-79	blood_patient19_t1	pneumonia	blood	M	none	positive	late
GSM830347	60-79	blood_patient20_t0	pneumonia	blood	M	none	positive	early
GSM830348	60-79	blood_patient20_t1	pneumonia	blood	M	none	positive	late

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