Sample Set Annotation Tool: CD14 and complement crosstalk and largely mediate the transcriptional response to Escherichia coli in human whole blood as revealed by DNA microarray - GSE55537 [Escherichia coli (E. coli) exposure]

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Systemic inflammation like in sepsis is still lacking specific diagnostic markers and effective therapeutics. The first line of defense against intruding pathogens and endogenous damage signals is pattern recognition by e.g., complement and Toll-like receptors (TLR). Combined inhibition of a key complement component (C3 and C5) and TLR-co-receptor CD14 has been shown to attenuate certain systemic inflammatory responses. Using DNA microarray and gene annotation analyses, we aimed to decipher the effect of combined inhibition of C3 and CD14 on the transcriptional response to bacterial challenge in human whole blood. Importantly, combined inhibition reversed the transcriptional changes of 70% of the 2335 genes which significantly responded to heat-inactivated Escherichia coli by on average 80%. Single inhibition was less efficient (p<0.001) but revealed a suppressive effect of C3 on 21% of the responding genes which was partially counteracted by CD14. Furthermore, CD14 dependency of the Escherichia coli-induced response was increased in C5-deficient compared to C5-sufficient blood. The observed crucial distinct and synergistic roles for complement and CD14 on the transcriptional level correspond to their broad impact on the inflammatory response in human blood, and their combined inhibition may become inevitable in the early treatment of acute systemic inflammation.

Hypothesis

Experimental Design

A microarray study was performed on whole blood from two healthy donors, a C5-deficient patient (C5D) (with and without C5-reconstitution) incubated for 120 min at 37oC with (i) PBS, (ii) E. coli or (iii) E. coli and inhibitors of complement (C3 or C5aR) and/or CD14 or inhibitor controls. The experiments were performed as biological replicates on two different days (healthy donor and C5D). Due to lack of a second C5-deficient patient, microarray analyses were prerformed twice (technical replicate) for C5D and day 1. Expression data in presence of PBS were used as reference for those in pressence of E. coli. Expression data in presence of E. coli were used as reference for those in pressence of E. coli and inhibitors. Expression data from 0 min incubation with PBS represent the initial state (T0). Whole blood was lysed in Applied Biosystems AB6100 total RNA chemistry lysis buffer and stored at -80oC prior to RNA extraction.

Experimental Variables

E. coli exposure (2021 ICD-10-CM code* = Not Applicable)

Controls

Methods

Robust Multichip Analysis by Partek® Genomics Suite software™ (transcripts with log2(signal value)<4 were excluded from the list)

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE55537

sufficent_none_none (S_N_N)

sufficent_1 x E06/mL E. coli_none (S_1M_E_N)

sufficent_5 x E06/mL E. coli_none (S_5M_E_N)

sufficent_1 x E06/mL E. coli_Compstatin (S_1M_E_C)

sufficent_5 x E06/mL E. coli_Compstatin (S_5M_E_C)

sufficent_1 x E06/mL E. coli_anti-CD14 (S_1M_E_aC)

sufficent_5 x E06/mL E. coli_anti-CD14 (S_5M_E_aC)

sufficent_1 x E06/mL E. coli_anti-CD14 and Compstatin (S_1M_E_aC_C)

sufficent_5 x E06/mL E. coli_anti-CD14 and Compstatin (S_5M_E_aC_C)

sufficent_1 x E06/mL E. coli_C5a receptor antagonist (S_1M_E_C5a)

sufficent_5 x E06/mL E. coli_C5a receptor antagonist (S_5M_E_C5a)

sufficent_1 x E06/mL E. coli_inhibitor controls (S_1M_E_In)

sufficent_5 x E06/mL E. coli_inhibitor controls (S_5M_E_In)

deficient_none_none (D_N_N)

deficient_1 x E06/mL E. coli_none (D_1M_E_N)

deficient_5 x E06/mL E. coli_none (D_5M_E_N)

deficient_1 x E06/mL E. coli_Compstatin (D_1M_E_C)

deficient_5 x E06/mL E. coli_Compstatin (D_5M_E_C)

deficient_1 x E06/mL E. coli_anti-CD14 (D_1M_E_aC)

deficient_5 x E06/mL E. coli_anti-CD14 (D_5M_E_aC)

deficient_1 x E06/mL E. coli_anti-CD14 and Compstatin (D_1M_E_aC_C)

deficient_5 x E06/mL E. coli_anti-CD14 and Compstatin (D_5M_E_aC_C)

deficient_1 x E06/mL E. coli_C5a receptor antagonist (D_1M_E_C5a)

deficient_5 x E06/mL E. coli_C5a receptor antagonist (D_5M_E_C5a)

deficient_1 x E06/mL E. coli_inhibitor controls (D_1M_E_In)

deficient_5 x E06/mL E. coli_inhibitor controls (D_5M_E_In)

C5 reconstituted_none_none (R_N_N)

C5 reconstituted_1 x E06/mL E. coli_none (R_1M_E_N)

C5 reconstituted_5 x E06/mL E. coli_none (R_5M_E_N)

C5 reconstituted_1 x E06/mL E. coli_Compstatin (R_1M_E_C)

C5 reconstituted_5 x E06/mL E. coli_Compstatin (R_5M_E_C)

C5 reconstituted_1 x E06/mL E. coli_anti-CD14 (R_1M_E_aC)

C5 reconstituted_5 x E06/mL E. coli_anti-CD14 (R_5M_E_aC)

C5 reconstituted_1 x E06/mL E. coli_anti-CD14 and Compstatin (R_1M_E_aC_C)

C5 reconstituted_5 x E06/mL E. coli_anti-CD14 and Compstatin (R_5M_E_aC_C)

C5 reconstituted_1 x E06/mL E. coli_C5a receptor antagonist (R_1M_E_C5a)

C5 reconstituted_5 x E06/mL E. coli_C5a receptor antagonist (R_5M_E_C5a)

C5 reconstituted_1 x E06/mL E. coli_inhibitor controls (R_1M_E_In)

C5 reconstituted_5 x E06/mL E. coli_inhibitor controls (R_5M_E_In)

Expected Samples:

73 Samples Loaded: 73

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

E. coli exposure

Treatment Protocol

Growth Protocol

Extraction Protocol

total RNA was isolated from whole blood using Applied Biosystems ABI PRISM™ 6100 Nucleic Acid PrepStation and AB6100 total RNA chemistry; RNA integrity with average RIN of 8.8 was verified by 2100 Bioanalyzer (Agilent).

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	tissue	c5	activator	genotype
GSM1338816	ctrl2_PBS_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 2
GSM1338817	ctrl2_PBS_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 2
GSM1338818	ctrl_PBS_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 1
GSM1338819	ctrl_PBS_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 1
GSM1338820	ctrl2_E.coli_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 2

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File
sampleset4000172_sampleannotations_GSE55537_new.csv Sample Set Spreadsheet
sampleset4000172_sampleannotations_GSE55537_new.csv Sample Set Spreadsheet

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All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Tissue	C5	Activator	Genotype	Inhibitor	C5 Activator V1	C5 Inhibitor V1	C5 Activator Inhibitor V1
GSM1338816	ctrl2_PBS_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 2	none	sufficent_none	sufficent_none	sufficent_none_none
GSM1338817	ctrl2_PBS_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 2	none	sufficent_none	sufficent_none	sufficent_none_none
GSM1338818	ctrl_PBS_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 1	none	sufficent_none	sufficent_none	sufficent_none_none
GSM1338819	ctrl_PBS_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 1	none	sufficent_none	sufficent_none	sufficent_none_none
GSM1338820	ctrl2_E.coli_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 2	none	sufficent_1 x E06/mL E. coli	sufficent_none	sufficent_1 x E06/mL E. coli_none
GSM1338821	ctrl2_E.coli_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 2	none	sufficent_5 x E06/mL E. coli	sufficent_none	sufficent_5 x E06/mL E. coli_none
GSM1338822	ctrl_E.coli_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 1	none	sufficent_1 x E06/mL E. coli	sufficent_none	sufficent_1 x E06/mL E. coli_none
GSM1338823	ctrl_E.coli_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 1	none	sufficent_5 x E06/mL E. coli	sufficent_none	sufficent_5 x E06/mL E. coli_none
GSM1338824	ctrl2_Compstatin_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 2	Compstatin	sufficent_1 x E06/mL E. coli	sufficent_Compstatin	sufficent_1 x E06/mL E. coli_Compstatin
GSM1338825	ctrl2_Compstatin_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 2	Compstatin	sufficent_5 x E06/mL E. coli	sufficent_Compstatin	sufficent_5 x E06/mL E. coli_Compstatin
GSM1338826	ctrl_Compstatin_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 1	Compstatin	sufficent_1 x E06/mL E. coli	sufficent_Compstatin	sufficent_1 x E06/mL E. coli_Compstatin
GSM1338827	ctrl_Compstatin_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 1	Compstatin	sufficent_5 x E06/mL E. coli	sufficent_Compstatin	sufficent_5 x E06/mL E. coli_Compstatin
GSM1338828	ctrl2_anti-CD14_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 2	anti-CD14	sufficent_1 x E06/mL E. coli	sufficent_anti-CD14	sufficent_1 x E06/mL E. coli_anti-CD14
GSM1338829	ctrl2_anti-CD14_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 2	anti-CD14	sufficent_5 x E06/mL E. coli	sufficent_anti-CD14	sufficent_5 x E06/mL E. coli_anti-CD14
GSM1338830	ctrl_anti-CD14_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 1	anti-CD14	sufficent_1 x E06/mL E. coli	sufficent_anti-CD14	sufficent_1 x E06/mL E. coli_anti-CD14
GSM1338831	ctrl_anti-CD14_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 1	anti-CD14	sufficent_5 x E06/mL E. coli	sufficent_anti-CD14	sufficent_5 x E06/mL E. coli_anti-CD14
GSM1338832	ctrl2_anti-CD14+Compstatin_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 2	anti-CD14 and Compstatin	sufficent_1 x E06/mL E. coli	sufficent_anti-CD14 and Compstatin	sufficent_1 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338833	ctrl2_anti-CD14+Compstatin_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 2	anti-CD14 and Compstatin	sufficent_5 x E06/mL E. coli	sufficent_anti-CD14 and Compstatin	sufficent_5 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338834	ctrl_anti-CD14+Compstatin_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 1	anti-CD14 and Compstatin	sufficent_1 x E06/mL E. coli	sufficent_anti-CD14 and Compstatin	sufficent_1 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338835	ctrl_anti-CD14+Compstatin_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 1	anti-CD14 and Compstatin	sufficent_5 x E06/mL E. coli	sufficent_anti-CD14 and Compstatin	sufficent_5 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338836	ctrl2_C5aR antagonist_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 2	C5a receptor antagonist	sufficent_1 x E06/mL E. coli	sufficent_C5a receptor antagonist	sufficent_1 x E06/mL E. coli_C5a receptor antagonist
GSM1338837	ctrl2_C5aR antagonist_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 2	C5a receptor antagonist	sufficent_5 x E06/mL E. coli	sufficent_C5a receptor antagonist	sufficent_5 x E06/mL E. coli_C5a receptor antagonist
GSM1338838	ctrl_C5aR antagonist_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 1	C5a receptor antagonist	sufficent_1 x E06/mL E. coli	sufficent_C5a receptor antagonist	sufficent_1 x E06/mL E. coli_C5a receptor antagonist
GSM1338839	ctrl_C5aR antagonist_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 1	C5a receptor antagonist	sufficent_5 x E06/mL E. coli	sufficent_C5a receptor antagonist	sufficent_5 x E06/mL E. coli_C5a receptor antagonist
GSM1338840	ctrl2_inhibitor ctrl_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 2	inhibitor controls	sufficent_1 x E06/mL E. coli	sufficent_inhibitor controls	sufficent_1 x E06/mL E. coli_inhibitor controls
GSM1338841	ctrl2_inhibitor ctrl_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 2	inhibitor controls	sufficent_5 x E06/mL E. coli	sufficent_inhibitor controls	sufficent_5 x E06/mL E. coli_inhibitor controls
GSM1338842	ctrl_inhibitor ctrl_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	1 x E06/mL E. coli	healthy donor 1	inhibitor controls	sufficent_1 x E06/mL E. coli	sufficent_inhibitor controls	sufficent_1 x E06/mL E. coli_inhibitor controls
GSM1338843	ctrl_inhibitor ctrl_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	5 x E06/mL E. coli	healthy donor 1	inhibitor controls	sufficent_5 x E06/mL E. coli	sufficent_inhibitor controls	sufficent_5 x E06/mL E. coli_inhibitor controls
GSM1338844	C5D_PBS_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	none	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	deficient_none	deficient_none	deficient_none_none
GSM1338845	C5D_PBS_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	none	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	deficient_none	deficient_none	deficient_none_none
GSM1338846	C5D_PBS_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	none	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	deficient_none	deficient_none	deficient_none_none
GSM1338847	C5D_E.coli_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	deficient_1 x E06/mL E. coli	deficient_none	deficient_1 x E06/mL E. coli_none
GSM1338848	C5D_E.coli_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	deficient_1 x E06/mL E. coli	deficient_none	deficient_1 x E06/mL E. coli_none
GSM1338849	C5D_E.coli_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	deficient_5 x E06/mL E. coli	deficient_none	deficient_5 x E06/mL E. coli_none
GSM1338850	C5D_Compstatin_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	Compstatin	deficient_1 x E06/mL E. coli	deficient_Compstatin	deficient_1 x E06/mL E. coli_Compstatin
GSM1338851	C5D_Compstatin_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	Compstatin	deficient_1 x E06/mL E. coli	deficient_Compstatin	deficient_1 x E06/mL E. coli_Compstatin
GSM1338852	C5D_Compstatin_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	Compstatin	deficient_5 x E06/mL E. coli	deficient_Compstatin	deficient_5 x E06/mL E. coli_Compstatin
GSM1338853	C5D_anti-CD14_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14	deficient_1 x E06/mL E. coli	deficient_anti-CD14	deficient_1 x E06/mL E. coli_anti-CD14
GSM1338854	C5D_anti-CD14_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14	deficient_1 x E06/mL E. coli	deficient_anti-CD14	deficient_1 x E06/mL E. coli_anti-CD14
GSM1338855	C5D_anti-CD14_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14	deficient_5 x E06/mL E. coli	deficient_anti-CD14	deficient_5 x E06/mL E. coli_anti-CD14
GSM1338856	C5D_anti-CD14+Compstatin_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14 and Compstatin	deficient_1 x E06/mL E. coli	deficient_anti-CD14 and Compstatin	deficient_1 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338857	C5D_anti-CD14+Compstatin_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14 and Compstatin	deficient_1 x E06/mL E. coli	deficient_anti-CD14 and Compstatin	deficient_1 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338858	C5D_anti-CD14+Compstatin_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14 and Compstatin	deficient_5 x E06/mL E. coli	deficient_anti-CD14 and Compstatin	deficient_5 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338859	C5D_C5aR antagonist_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	C5a receptor antagonist	deficient_1 x E06/mL E. coli	deficient_C5a receptor antagonist	deficient_1 x E06/mL E. coli_C5a receptor antagonist
GSM1338860	C5D_C5aR antagonist_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	C5a receptor antagonist	deficient_1 x E06/mL E. coli	deficient_C5a receptor antagonist	deficient_1 x E06/mL E. coli_C5a receptor antagonist
GSM1338861	C5D_C5aR antagonist_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	C5a receptor antagonist	deficient_5 x E06/mL E. coli	deficient_C5a receptor antagonist	deficient_5 x E06/mL E. coli_C5a receptor antagonist
GSM1338862	C5D_inhibitor ctrl_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	inhibitor controls	deficient_1 x E06/mL E. coli	deficient_inhibitor controls	deficient_1 x E06/mL E. coli_inhibitor controls
GSM1338863	C5D_inhibitor ctrl_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	inhibitor controls	deficient_1 x E06/mL E. coli	deficient_inhibitor controls	deficient_1 x E06/mL E. coli_inhibitor controls
GSM1338864	C5D_inhibitor ctrl_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	inhibitor controls	deficient_5 x E06/mL E. coli	deficient_inhibitor controls	deficient_5 x E06/mL E. coli_inhibitor controls
GSM1338865	C5DR_PBS_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	none	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	C5 reconstituted_none	C5 reconstituted_none	C5 reconstituted_none_none
GSM1338866	C5DR_PBS_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	none	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	C5 reconstituted_none	C5 reconstituted_none	C5 reconstituted_none_none
GSM1338867	C5DR_PBS_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	none	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	C5 reconstituted_none	C5 reconstituted_none	C5 reconstituted_none_none
GSM1338868	C5DR_E.coli_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_none	C5 reconstituted_1 x E06/mL E. coli_none
GSM1338869	C5DR_E.coli_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_none	C5 reconstituted_1 x E06/mL E. coli_none
GSM1338870	C5DR_E.coli_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	C5 reconstituted_5 x E06/mL E. coli	C5 reconstituted_none	C5 reconstituted_5 x E06/mL E. coli_none
GSM1338871	C5DR_Compstatin_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	Compstatin	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_Compstatin	C5 reconstituted_1 x E06/mL E. coli_Compstatin
GSM1338872	C5DR_Compstatin_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	Compstatin	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_Compstatin	C5 reconstituted_1 x E06/mL E. coli_Compstatin
GSM1338873	C5DR_Compstatin_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	Compstatin	C5 reconstituted_5 x E06/mL E. coli	C5 reconstituted_Compstatin	C5 reconstituted_5 x E06/mL E. coli_Compstatin
GSM1338874	C5DR_anti-CD14_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_anti-CD14	C5 reconstituted_1 x E06/mL E. coli_anti-CD14
GSM1338875	C5DR_anti-CD14_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_anti-CD14	C5 reconstituted_1 x E06/mL E. coli_anti-CD14
GSM1338876	C5DR_anti-CD14_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14	C5 reconstituted_5 x E06/mL E. coli	C5 reconstituted_anti-CD14	C5 reconstituted_5 x E06/mL E. coli_anti-CD14
GSM1338877	C5DR_anti-CD14+Compstatin_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14 and Compstatin	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_anti-CD14 and Compstatin	C5 reconstituted_1 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338878	C5DR_anti-CD14+Compstatin_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14 and Compstatin	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_anti-CD14 and Compstatin	C5 reconstituted_1 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338879	C5DR_anti-CD14+Compstatin_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	anti-CD14 and Compstatin	C5 reconstituted_5 x E06/mL E. coli	C5 reconstituted_anti-CD14 and Compstatin	C5 reconstituted_5 x E06/mL E. coli_anti-CD14 and Compstatin
GSM1338880	C5DR_C5aR antagonist_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	C5a receptor antagonist	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_C5a receptor antagonist	C5 reconstituted_1 x E06/mL E. coli_C5a receptor antagonist
GSM1338881	C5DR_C5aR antagonist_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	C5a receptor antagonist	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_C5a receptor antagonist	C5 reconstituted_1 x E06/mL E. coli_C5a receptor antagonist
GSM1338882	C5DR_C5aR antagonist_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	C5a receptor antagonist	C5 reconstituted_5 x E06/mL E. coli	C5 reconstituted_C5a receptor antagonist	C5 reconstituted_5 x E06/mL E. coli_C5a receptor antagonist
GSM1338883	C5DR_inhibitor ctrl_D1	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	inhibitor controls	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_inhibitor controls	C5 reconstituted_1 x E06/mL E. coli_inhibitor controls
GSM1338884	C5DR_inhibitor ctrl_D1_No2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	1 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	inhibitor controls	C5 reconstituted_1 x E06/mL E. coli	C5 reconstituted_inhibitor controls	C5 reconstituted_1 x E06/mL E. coli_inhibitor controls
GSM1338885	C5DR_inhibitor ctrl_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	C5 reconstituted	5 x E06/mL E. coli	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	inhibitor controls	C5 reconstituted_5 x E06/mL E. coli	C5 reconstituted_inhibitor controls	C5 reconstituted_5 x E06/mL E. coli_inhibitor controls
GSM1338886	ctrl2_T0_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 2	none	sufficent_none	sufficent_none	sufficent_none_none
GSM1338887	ctrl_T0_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	sufficent	none	healthy donor 1	none	sufficent_none	sufficent_none	sufficent_none_none
GSM1338888	C5D_T0_D2	fresh venous blood anticoagulated with 50 µg/mL thrombin-inhibitor lepirudin	deficient	none	C5 deficient patient with two aberrant C5 mRNA products with deletions of exon 27 and exons 26 and 27, respectively	none	deficient_none	deficient_none	deficient_none_none

Group Sets View in Gene Expression Browser

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C5 Activator		View
C5 Activator Inhibitor v1		View
C5 Inhibitor v1	Default	View
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Module Analysis

Group Set
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C5 Activator
C5 Activator Inhibitor v1
C5 Inhibitor v1

SIDRA