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Clinical

Identification of biomarkers of response to IFNg during endotoxin tolerance: application to septic shock - GSE46914

Purpose

The rapid development in septic patients of features of marked immunosuppression associated with increased risk of nosocomial infections and mortality represents the rational ...

Hypothesis

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Experimental Design

PBMCs from 6 healthy donor were left unstimulated (medium) or with 1 shot of LPS (LPS unprimed), 2 shots of LPS (LPS primed) or 2 shots of LPS and Interferon gamma (LPS ...

Experimental Variables

Lipopolysaccharide exposure (2021 ICD-10-CM code* = Not Applicable)

Controls

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Methods

Expression data were generated using the Robust Multi-array Average (RMA) method implemented in the Affymetrix package of the Bioconductor microarray analysis environment ...

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE46914

Microarray
Affymetrix HG-U133_Plus_2
24 Samples Loaded: 24
Human (Homo sapiens)
PBMC
Lipopolysaccharide exposure
Sample Set Spreadsheet
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Samples Preview
Sample ID !Sample_title culture condition
GSM1141035 PBMCs_HV2_medium medium
GSM1141036 PBMCs_HV2_LPS unprimed LPS unprimed
GSM1141037 PBMCs_HV2_LPS primed LPS primed
GSM1141038 PBMCs_HV2_LPS primed+IFNg LPS primed + IFNg
GSM1141039 PBMCs_HV3_medium medium
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Log2 Transformed
Raw Signal
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Sample ID !Sample Title Culture condition
GSM1141035
PBMCs_HV2_medium
medium
GSM1141036
PBMCs_HV2_LPS unprimed
LPS unprimed
GSM1141037
PBMCs_HV2_LPS primed
LPS primed
GSM1141038
PBMCs_HV2_LPS primed+IFNg
LPS primed + IFNg
GSM1141039
PBMCs_HV3_medium
medium
GSM1141040
PBMCs_HV3_LPS unprimed
LPS unprimed
GSM1141041
PBMCs_HV3_LPS primed
LPS primed
GSM1141042
PBMCs_HV3_LPS primed+IFNg
LPS primed + IFNg
GSM1141043
PBMCs_HV5_medium
medium
GSM1141044
PBMCs_HV5_LPS unprimed
LPS unprimed
GSM1141045
PBMCs_HV5_LPS primed
LPS primed
GSM1141046
PBMCs_HV5_LPS primed+IFNg
LPS primed + IFNg
GSM1141047
PBMCs_HV6_medium
medium
GSM1141048
PBMCs_HV6_LPS unprimed
LPS unprimed
GSM1141049
PBMCs_HV6_LPS primed
LPS primed
GSM1141050
PBMCs_HV6_LPS primed+IFNg
LPS primed + IFNg
GSM1141051
PBMCs_HV7_medium
medium
GSM1141052
PBMCs_HV7_LPS unprimed
LPS unprimed
GSM1141053
PBMCs_HV7_LPS primed
LPS primed
GSM1141054
PBMCs_HV7_LPS primed+IFNg
LPS primed + IFNg
GSM1141055
PBMCs_HV9_medium
medium
GSM1141056
PBMCs_HV9_LPS unprimed
LPS unprimed
GSM1141057
PBMCs_HV9_LPS primed
LPS primed
GSM1141058
PBMCs_HV9_LPS primed+IFNg
LPS primed + IFNg

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