Normal children, children with SIRS, children with sepsis, and children with septic shock.Objectives: To advance our biological understanding of pediatric septic shock, we measured the genome-level expression profiles of critically ill children representing the systemic inflammatory response syndrome (SIRS), sepsis, and septic shock spectrum.

Prospective observational study involving microarray-based bioinformatics.

Systemic inflammatory response syndrome (2021 ICD-10-CM code* = R65.1)

Sepsis (2021 ICD-10-CM code* = A41)

Septic shock (2021 ICD-10-CM code* = R65.21)

Commercial Affymetrix Human Genome U133 Plus 2.0 GeneChip (Affymetrix, Santa Clara, CA). GeneChip CEL files were subjected to RMA normalization using the GeneSpring GX 7.1.Standard Affymetrix internal control genes were used to check the quality of the assay by the signals of the 3' probe set to the 5' probe set of the internal control genes, GAPDH and beta-actin, with acceptable 3' to 5' ratios between 1 and 3. Eukaryotic Spike controls were used to determine that the hybridization of target RNA to the array occurred properly.GeneSpring 7.2 (Agilent technologies Inc. Palo Alto, California) was used for normalization, clustering, filtering, and statistical analyses. The Raw CEL files were processed using the RMA (Robust Multichip Average) built in GeneSpring software. All the samples were then normalized to the median of the controls.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE13904