Sample Set Annotation Tool: Peripheral blood cells expression data form 7 patients with severe pdm (H1N1) influensa and 7 gender and age matched healthy controls - GSE27131 [Influenza virus H1N1, Influenza (A/H1N1 new subtype), Influenza A virus subtype H1N1, Septic Influenza]

Study Design
Assay
Sample Info
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Info
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Analysis

Purpose

Using PAXgene tubes, peripheral blood samples were collected from seven patients >18 years with documented pdm(H1N1) influenza, bilateral chest infiltrates, and in need of ventilation support. Significant co-morbidity was exclusion criterion. Expression profiles were compared with 7 age matched controls. Using a false discovery rate < 5% and an absolute fold change > 2, 370 genes were differentially expressed in case and controls.A second sample was collected after ca 6 days in the 7 patients and temporal changes in expression profiles investigated. Over expressed putative mediators of inflammatory lung dammage were measured on the protein level.

Hypothesis

Experimental Design

14 patient samples (7 paired samples) and 7 control samples were performed.

Experimental Variables

Controls

Methods

Raw data were processed using Partek Genomics Suite v. 6.5 to generate RMA normalised intensities. Differential expression was assessed using One-way or Two-way ANOVA in the Partek software dependent of if the samples were paired or not.

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27131

Expected Samples:

21 Samples Loaded: 21

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

Influenza virus (H1N1)

Treatment Protocol

Blood samples were collected with PAXgene Blood RNA tubes

Growth Protocol

Peripheral blood cells

Extraction Protocol

RNA extraction was performed with PAXgene Blood RNA kit according to manufactors instructions, and RNA quality assesed with Bioanalyzer

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	Gender	tissue	Grouping	Age (years)
GSM670449	blood_day0_patient1	male	blood	Septic Influenza	54
GSM670450	blood_day6_patient1	male	blood	Septic Influenza	54
GSM670451	blood_day0_patient2	male	blood	Septic Influenza	38
GSM670452	blood_day6_patient2	male	blood	Septic Influenza	38
GSM670453	blood_day0_patient3	male	blood	Septic Influenza	59

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File
sampleset4000228_sampleannotations.csv Sample Set Spreadsheet
sampleset4000228_sampleannotations.csv Sample Set Spreadsheet
sampleset4000228_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Gender	Tissue	Grouping	Age (years)	Patient	Timepoints	Control
GSM670449	blood_day0_patient1	male	blood	Septic Influenza	54	1	Septic Influenza Day 0
GSM670450	blood_day6_patient1	male	blood	Septic Influenza	54	1	Septic Influenza Day 6
GSM670451	blood_day0_patient2	male	blood	Septic Influenza	38	2	Septic Influenza Day 0
GSM670452	blood_day6_patient2	male	blood	Septic Influenza	38	2	Septic Influenza Day 6
GSM670453	blood_day0_patient3	male	blood	Septic Influenza	59	3	Septic Influenza Day 0
GSM670454	blood_day6_patient3	male	blood	Septic Influenza	59	3	Septic Influenza Day 6
GSM670455	blood_day0_patient4	female	blood	Septic Influenza	28	4	Septic Influenza Day 0
GSM670456	blood_day6_patient4	female	blood	Septic Influenza	28	4	Septic Influenza Day 6
GSM670457	blood_day0_patient5	male	blood	Septic Influenza	38	5	Septic Influenza Day 0
GSM670458	blood_day6_patient5	male	blood	Septic Influenza	38	5	Septic Influenza Day 6
GSM670459	blood_day0_patient6	male	blood	Septic Influenza	46	6	Septic Influenza Day 0
GSM670460	blood_day6_patient6	male	blood	Septic Influenza	46	6	Septic Influenza Day 6
GSM670461	blood_day0_patient7	male	blood	Septic Influenza	25	7	Septic Influenza Day 0
GSM670462	blood_day6_patient7	male	blood	Septic Influenza	25	7	Septic Influenza Day 6
GSM670463	Blood_Control 1	male	blood	Healthy Control	41		Healthy Control	1
GSM670464	Blood_Control 2	male	blood	Healthy Control	48		Healthy Control	2
GSM670465	Blood_Control 3	female	blood	Healthy Control	38		Healthy Control	3
GSM670466	Blood_Control 4	male	blood	Healthy Control	26		Healthy Control	4
GSM670467	Blood_Control 5	male	blood	Healthy Control	41		Healthy Control	5
GSM670468	Blood_Control 6	female	blood	Healthy Control	40		Healthy Control	6
GSM670469	Blood_Control 7	female	blood	Healthy Control	35		Healthy Control	7

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