Sample Set Annotation Tool: Transcriptomic profiles of blood in murine polymicrobial sepsis

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Transcriptional effects in circulating leukocytes from mice challenged to peritoneal contamination and infection (PCI) compared to sham (vehicle control).We gratefully acknowledge the BMBF grant for the Center for Sepsis Control and Care (CSCC) for this study.

Hypothesis

Experimental Design

Time resolved murine PCI challenge groups x four replicates, Sham control x three replicates.

Experimental Variables

Controls

Methods

Gene chips were scanned and pre-processed using BeadStudio (version 3.23). Data analysis was carried out using R statistics software (version 2.92) and BioConductor (version 2.4.1).

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE36608

Expected Samples:

11 Samples Loaded: 11

Species

Mouse (Mus musculus)

Sample Source

Whole Blood

Disease

Sepsis

Treatment Protocol

Intraperitoneal injection of 200 µl of a human faeces suspension (KBE 75 x 106/ml) leading to peritoneal contamination and infection (PCI) in mice. Sham controls received intraperitoneal injection of 200 µl of saline.

Growth Protocol

8 weeks old female C57/BL6 Jackson mice.

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	Gender	treatment	strain	Age (weeks)
GSM897473	Blood-Sham-6h-Rep1	Female	sham treatment	C57/BL6 Jackson mice	8
GSM897474	Blood-Sham-6h-Rep2	Female	sham treatment	C57/BL6 Jackson mice	8
GSM897475	Blood-Sham-6h-Rep3	Female	sham treatment	C57/BL6 Jackson mice	8
GSM897476	Blood-PCI-6h-Rep1	Female	challenged by PCI after 6 hours	C57/BL6 Jackson mice	8
GSM897477	Blood-PCI-6h-Rep2	Female	challenged by PCI after 6 hours	C57/BL6 Jackson mice	8

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File
sampleset4000231_sampleannotations.csv Sample Set Spreadsheet
sampleset4000231_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Gender	Treatment	Strain	Age (weeks)
GSM897473	Blood-Sham-6h-Rep1	Female	sham treatment	C57/BL6 Jackson mice	8
GSM897474	Blood-Sham-6h-Rep2	Female	sham treatment	C57/BL6 Jackson mice	8
GSM897475	Blood-Sham-6h-Rep3	Female	sham treatment	C57/BL6 Jackson mice	8
GSM897476	Blood-PCI-6h-Rep1	Female	challenged by PCI after 6 hours	C57/BL6 Jackson mice	8
GSM897477	Blood-PCI-6h-Rep2	Female	challenged by PCI after 6 hours	C57/BL6 Jackson mice	8
GSM897478	Blood-PCI-6h-Rep3	Female	challenged by PCI after 6 hours	C57/BL6 Jackson mice	8
GSM897479	Blood-PCI-6h-Rep4	Female	challenged by PCI after 6 hours	C57/BL6 Jackson mice	8
GSM897480	Blood-PCI-24h-Rep1	Female	challenged by PCI after 24 hours	C57/BL6 Jackson mice	8
GSM897481	Blood-PCI-24h-Rep2	Female	challenged by PCI after 24 hours	C57/BL6 Jackson mice	8
GSM897482	Blood-PCI-24h-Rep3	Female	challenged by PCI after 24 hours	C57/BL6 Jackson mice	8
GSM897483	Blood-PCI-24h-Rep4	Female	challenged by PCI after 24 hours	C57/BL6 Jackson mice	8

Group Sets View in Gene Expression Browser

Name
All Samples		View
Treatment	Default	View
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Module Analysis

Group Set
All Samples
Treatment

SIDRA