Sample Set Annotation Tool: In vitro: Whole blood stimulation- GSE30101

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

Genome-wide profiling of whole blood from healthy adult volunteers before and after receiving non-live vaccines including seasonal influenza or pneumococcal vaccine or placebo (saline) injections. This SuperSeries is composed of the following SubSeries:

GSE30059	Genome-wide profiling of whole blood from healthy adult volunteers before and after receiving non-live vaccines including seasonal influenza or pneumococcal vaccine or placebo (saline) injections I
GSE48762	Genome-wide profiling of whole blood from healthy adult volunteers before and after receiving non-live vaccines including seasonal influenza or pneumococcal vaccine or placebo (saline) injections II

Hypothesis

Experimental Design

Refer to individual Series

Experimental Variables

Controls

Methods

Following background subtraction, the raw signal intensities extracted with Illumina Genome Studio v2010.1 Gene Expression 1.6.0 software were scaled to the mean intensity of the dataset.

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30101

Expected Samples:

80 Samples Loaded: 80

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

In-vitro Whole Blood Stimulation

Treatment Protocol

Growth Protocol

Extraction Protocol

RNA was isolated using the MagMAX™-96 Blood RNA Isolation Kit (Applied Biosystems/Ambion, Austin, TX, USA) according to the manufacturer's instructions

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	Age	Gender	donor	Culture condition	Array Sample ID
4809000019_C	22	F	HD0063	HKE.coli	27116
4809000019_D	22	F	HD0063	Flu	27117
4809000019_G	41	M	HD0061	IFNa2b	27118
4809000019_I	32	F	HD0055	PAM3	27119
4809000019_J	41	M	HD0061	HKAL	27120

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File
sampleset296_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	Age	Gender	Donor	Culture condition	Array Sample ID	Group Set	ADAM9	CXCL10
4809000019_C	22	F	HD0063	HKE.coli	27116	HK E.coli	26.396	23330.8
4809000019_D	22	F	HD0063	Flu	27117	Flu	17.33036	277.3267
4809000019_G	41	M	HD0061	IFNa2b	27118	IFNa2b	27.29878	220.6637
4809000019_I	32	F	HD0055	PAM3	27119	PAM3	47.86802	121.2552
4809000019_J	41	M	HD0061	HKAL	27120	HKAL	24.74811	3374.449
4809000021_A	40	F	HD0064	IFNa2b	27121	IFNa2b	26.46074	436.825
4809000021_K	41	M	HD0061	IFNb	27122	IFNb	32.03705	37.32098
4809000025_B	41	M	HD0061	Zymosan	27123	Zymosan	20.86564	36.71877
4809000025_C	32	F	HD0055	RSV	27124	RSV	27.97968	1780.935
4809000025_E	22	F	HD0063	E-LPS	27125	E-LPS	16.85201	17465.13
4809000025_H	40	F	HD0064	HKE.coli	27126	HK E.coli	41.38318	121.969
4809000025_I	22	F	HD0063	Ex-vivo control	27127	Ex-vivo	20.58604	20705.6
4809000025_L	40	F	HD0064	R837	27128	R837	37.69903	131.7569
4809000026_G	32	F	HD0055	Non-stimulated	27129	Control	41.82703	79.6393
4809000026_K	41	M	HD0061	CpG type A	27130	CpG	53.09872	9989.634
4809000026_L	40	F	HD0064	CpG type A	27131	CpG	32.24323	273.9563
4809000027_F	32	F	HD0055	E-LPS	27132	E-LPS	48.24205	164.8602
4809000027_I	41	M	HD0061	IL-18	27133	IL-18	24.90874	42.22706
4809000028_A	41	M	HD0061	TNFa	27134	TNFa	23.59606	199.6931
4809000028_C	32	F	HD0055	Flu	27135	Flu	39.07818	350.1708
4809000028_D	40	F	HD0064	Zymosan	27136	Zymosan	62.62659	73.06765
4809000028_F	40	F	HD0064	IL-18	27137	IL-18	54.17403	12759.81
4809000028_H	41	M	HD0061	Flagellin	27138	Flagellin	48.50327	0.13527
4809000028_I	22	F	HD0063	PAM3	27139	PAM3	27.933	25687.74
4809000028_J	22	F	HD0063	IFNg	27140	IFNg	30.70194	6347.038
4809000028_L	41	M	HD0061	HKE.coli	27141	HK E.coli	33.72829	20125.87
4809000029_A	40	F	HD0064	Flagellin	27142	Flagellin	58.80812	2421.057
4809000029_B	40	F	HD0064	TNFa	27143	TNFa	26.5444	30195.04
4809000029_C	22	F	HD0063	Non-stimulated	27144	Control	17.32637	5163.599
4809000029_G	41	M	HD0061	HKSA	27145	HKSA	98.73809	72.60593
4809000029_H	41	M	HD0061	R837	27146	R837	28.67905	19979.75
4809000029_I	40	F	HD0064	IFNb	27147	IFNb	19.42603	886.2922
4809000029_J	22	F	HD0063	HKLP	27148	HKLP	101.5273	18612.14
4809000029_K	32	F	HD0055	HKLP	27149	HKLP	43.12257	12.31542
4809000029_L	32	F	HD0055	IFNg	27150	IFNg	24.02608	1433.834
4809000053_B	40	F	HD0064	PolyIC	27151	PolyIC	35.778	2804.063
4809000053_D	41	M	HD0061	PolyIC	27152	PolyIC	64.06626	27538.24
4809000053_F	22	F	HD0063	RSV	27153	RSV	16.68216	15204.64
4809000053_I	40	F	HD0064	HKSA	27154	HKSA	36.61574	2070.083
4809000053_K	40	F	HD0064	HKAL	27155	HKAL	61.22818	105.3258
4809000031_A	32	F	HD0055	IFNa2b	27156	IFNa2b	90.39224	4381.029
4809000031_C	32	F	HD0055	PolyIC	27157	PolyIC	39.30614	1826.292
4809000031_E	41	M	HD0061	Ex-vivo control	27158	Ex-vivo	39.41807	173.811
4809000031_F	22	F	HD0063	HKSA	27159	HKSA	40.80621	988.0381
4809000031_L	40	F	HD0064	PAM3	27160	PAM3	30.92645	4.018568
4809000032_A	32	F	HD0055	HKAL	27161	HKAL	31.30517	349.4028
4809000032_B	22	F	HD0063	HKAL	27162	HKAL	31.90643	18028.73
4809000032_F	22	F	HD0063	CpG type A	27163	CpG	16.00819	2632.458
4809000032_H	32	F	HD0055	IL-18	27164	IL-18	22.58402	18254.43
4809000032_I	40	F	HD0064	E-LPS	27165	E-LPS	50.88025	162.5212
4809000032_J	22	F	HD0063	R837	27166	R837	52.54627	152.8236
4809000032_L	40	F	HD0064	Flu	27167	Flu	78.35494	91.79517
4809000033_A	40	F	HD0064	Ex-vivo control	27168	Ex-vivo	47.42824	77.1902
4809000033_B	22	F	HD0063	IFNb	27169	IFNb	30.33253	2364.093
4809000033_C	32	F	HD0055	TNFa	27170	TNFa	12.85606	64.29774
4809000033_D	22	F	HD0063	Zymosan	27171	Zymosan	45.33032	98.8005
4809000033_G	32	F	HD0055	HKE.coli	27172	HK E.coli	59.0194	2648.524
4809000044_D	41	M	HD0061	RSV	27173	RSV	11.98122	490.2601
4809000044_E	41	M	HD0061	IFNg	27174	IFNg	22.30635	31245.85
4809000044_F	22	F	HD0063	IL-18	27175	IL-18	100.6791	172.0786
4809000044_J	32	F	HD0055	Ex-vivo control	27176	Ex-vivo	40.31347	9.363182
4809000045_B	40	F	HD0064	HKLP	27177	HKLP	4.080217	23860.14
4809000045_C	41	M	HD0061	E-LPS	27178	E-LPS	52.60559	2517.089
4809000045_D	32	F	HD0055	IFNb	27179	IFNb	33.72153	18.72909
4809000045_E	32	F	HD0055	HKSA	27180	HKSA	72.54818	61.88761
4809000045_F	41	M	HD0061	PAM3	27181	PAM3	11.13848	144.8707
4809000045_K	32	F	HD0055	R837	27182	R837	71.53806	4803.507
4809000047_A	32	F	HD0055	Zymosan	27183	Zymosan	40.41602	12765.99
4809000047_B	22	F	HD0063	IFNa2b	27184	IFNa2b	60.32454	56.97206
4809000047_E	22	F	HD0063	PolyIC	27185	PolyIC	108.9123	38.25194
4809000047_H	40	F	HD0064	IFNg	27186	IFNg	38.90775	150.3708
4809000047_I	32	F	HD0055	CpG type A	27187	CpG	35.24175	16229.11
4809000047_K	41	M	HD0061	Non-stimulated	27188	Control	17.65964	4988.536
4809000047_L	32	F	HD0055	Flagellin	27189	Flagellin	37.44581	29333.81
4809000049_C	41	M	HD0061	HKLP	27190	HKLP	39.85216	206.6589
4809000049_E	22	F	HD0063	Flagellin	27191	Flagellin	34.45001	6412.662
4809000049_H	40	F	HD0064	Non-stimulated	27192	Control	27.02662	43.13696
4809000049_I	22	F	HD0063	TNFa	27193	TNFa	30.83655	8495.872
4809000049_J	40	F	HD0064	RSV	27194	RSV	128.5792	10.32956
4809000049_L	41	M	HD0061	Flu	27195	Flu	60.25828	3032.987

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