Sample Set Annotation Tool: Inflammasome-regulated cytokines are critical mediators of lung injury in humans - GSE32707 [Systemic inflammatory response syndrome (SIRS), Sepsis, Acute respiratory distress syndrome due to sepsis (ARDS)]

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

A microarray analysis involving whole blood samples isolated from critically ill patients in the medical intensive care unit at Brigham and Women's Hospital. Four groups of intubated subjects undergoing mechanical ventilation were recruited for the study: those with sepsis alone (Sepsis), those with sepsis + ARDS (se/ARDS), those with SIRS (SIRS), and those whithout sepsis, SIRS, or ARDS (untreated). Blood was obtained from patients on the day of admission (day 0) and 7 days later. RNA was isolated from the whole blood samples and microarrays were prepared to determine differential gene expression between the four groups.

Hypothesis

Experimental Design

Total RNA obtained from whole blood samples of critically ill patients

Experimental Variables

Systemic inflammatory response syndrome (2021 ICD-10-CM code* = R65.1)

Sepsis (2021 ICD-10-CM code* = A41)

Acute respiratory distress syndrome due to sepsis (2021 ICD-10-CM code* = J80-A41)

Controls

Methods

The data were normalised using quantile normalisation with GenomeStudio

Additional Information

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32707

Expected Samples:

144 Samples Loaded: 144

Species

Human (Homo sapiens)

Sample Source

Whole Blood

Disease

Systemic inflammatory response syndrome, sepsis, Acute respiratory distress syndrome due to sepsis

Treatment Protocol

Growth Protocol

Extraction Protocol

Total RNA was extracted using Qiagen's PAXgene Blood RNA Kit

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	tissue	Grouping	Subject id	Time series
GSM812609	sample 1_Sepsis Day 0	whole blood	Sepsis	170433	Sepsis Day 0
GSM812610	sample 2_Sepsis Day 7	whole blood	Sepsis	169921	Sepsis Day 7
GSM812611	sample 3_untreated	whole blood	untreated	169694	untreated
GSM812612	sample 4_untreated	whole blood	untreated	169695	untreated
GSM812613	sample 5_untreated	whole blood	untreated	169696	untreated

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File
sampleset4000178_sampleannotations (1).csv Sample Set Spreadsheet
sampleset4000178_sampleannotations (1).csv Sample Set Spreadsheet
sampleset4000178_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

Sample ID	!Sample Title	Tissue	Grouping	Subject id	Time series
GSM812609	sample 1_Sepsis Day 0	whole blood	Sepsis	170433	Sepsis Day 0
GSM812610	sample 2_Sepsis Day 7	whole blood	Sepsis	169921	Sepsis Day 7
GSM812611	sample 3_untreated	whole blood	untreated	169694	untreated
GSM812612	sample 4_untreated	whole blood	untreated	169695	untreated
GSM812613	sample 5_untreated	whole blood	untreated	169696	untreated
GSM812614	sample 6_Sepsis Day 0	whole blood	Sepsis	169930	Sepsis Day 0
GSM812615	sample 7_se/ARDS Day 0	whole blood	se/ARDS	169832	se/ARDS Day 0
GSM812616	sample 8_se/ARDS Day 0	whole blood	se/ARDS	169792	se/ARDS Day 0
GSM812617	sample 9_se/ARDS Day 7	whole blood	se/ARDS	170235	se/ARDS Day 7
GSM812618	sample 10_se/ARDS Day 0	whole blood	se/ARDS	169790	se/ARDS Day 0
GSM812619	sample 11_untreated	whole blood	untreated	169697	untreated
GSM812620	sample 12_untreated	whole blood	untreated	169698	untreated
GSM812621	sample 13_untreated	whole blood	untreated	169699	untreated
GSM812622	sample 14_Sepsis Day 0	whole blood	Sepsis	169926	Sepsis Day 0
GSM812623	sample 15_Sepsis Day 7	whole blood	Sepsis	169922	Sepsis Day 7
GSM812624	sample 16_untreated	whole blood	untreated	169701	untreated
GSM812625	sample 17_untreated	whole blood	untreated	169701	untreated
GSM812626	sample 18_SIRS Day 0	whole blood	SIRS	169936	SIRS Day 0
GSM812627	sample 19_untreated	whole blood	untreated	169700	untreated
GSM812628	sample 20_SIRS Day 0	whole blood	SIRS	169963	SIRS Day 0
GSM812629	sample 21_Sepsis Day 0	whole blood	Sepsis	169894	Sepsis Day 0
GSM812630	sample 22_Sepsis Day 7	whole blood	Sepsis	169932	Sepsis Day 7
GSM812631	sample 23_se/ARDS Day 0	whole blood	se/ARDS	169774	se/ARDS Day 0
GSM812632	sample 24_se/ARDS Day 7	whole blood	se/ARDS	169831	se/ARDS Day 7
GSM812633	sample 25_Sepsis Day 0	whole blood	Sepsis	169928	Sepsis Day 0
GSM812634	sample 26_Sepsis Day 0	whole blood	Sepsis	169853	Sepsis Day 0
GSM812635	sample 27_Sepsis Day 7	whole blood	Sepsis	169923	Sepsis Day 7
GSM812636	sample 28_SIRS Day 0	whole blood	SIRS	169982	SIRS Day 0
GSM812637	sample 29_SIRS Day 0	whole blood	SIRS	169935	SIRS Day 0
GSM812638	sample 30_Sepsis Day 0	whole blood	Sepsis	169929	Sepsis Day 0
GSM812639	sample 31_Sepsis Day 7	whole blood	Sepsis	170373	Sepsis Day 7
GSM812640	sample 32_SIRS Day 0	whole blood	SIRS	169987	SIRS Day 0
GSM812641	sample 33_SIRS Day 0	whole blood	SIRS	169965	SIRS Day 0
GSM812642	sample 34_Sepsis Day 0	whole blood	Sepsis	169925	Sepsis Day 0
GSM812643	sample 35_Sepsis Day 7	whole blood	Sepsis	169896	Sepsis Day 7
GSM812644	sample 36_SIRS Day 0	whole blood	SIRS	169957	SIRS Day 0
GSM812645	sample 37_Sepsis Day 0	whole blood	Sepsis	169897	Sepsis Day 0
GSM812646	sample 38_Sepsis Day 7	whole blood	Sepsis	170239	Sepsis Day 7
GSM812647	sample 39_se/ARDS Day 0	whole blood	se/ARDS	169791	se/ARDS Day 0
GSM812648	sample 40_se/ARDS Day 7	whole blood	se/ARDS	169775	se/ARDS Day 7
GSM812649	sample 41_Sepsis Day 0	whole blood	Sepsis	169898	Sepsis Day 0
GSM812650	sample 42_Sepsis Day 7	whole blood	Sepsis	170244	Sepsis Day 7
GSM812651	sample 43_Sepsis Day 0	whole blood	Sepsis	169899	Sepsis Day 0
GSM812652	sample 44_Sepsis Day 7	whole blood	Sepsis	170196	Sepsis Day 7
GSM812653	sample 45_Sepsis Day 0	whole blood	Sepsis	169913	Sepsis Day 0
GSM812654	sample 46_Sepsis Day 7	whole blood	Sepsis	170194	Sepsis Day 7
GSM812655	sample 47_untreated	whole blood	untreated	169722	untreated
GSM812656	sample 48_SIRS Day 0	whole blood	SIRS	169968	SIRS Day 0
GSM812657	sample 49_SIRS Day 0	whole blood	SIRS	169968	SIRS Day 0
GSM812658	sample 50_SIRS Day 0	whole blood	SIRS	169970	SIRS Day 0
GSM812659	sample 51_untreated	whole blood	untreated	169720	untreated
GSM812660	sample 52_Sepsis Day 0	whole blood	Sepsis	169914	Sepsis Day 0
GSM812661	sample 53_Sepsis Day 7	whole blood	Sepsis	170191	Sepsis Day 7
GSM812662	sample 54_Sepsis Day 0	whole blood	Sepsis	169915	Sepsis Day 0
GSM812663	sample 55_Sepsis Day 7	whole blood	Sepsis	170238	Sepsis Day 7
GSM812664	sample 56_Sepsis Day 0	whole blood	Sepsis	170380	Sepsis Day 0
GSM812665	sample 57_Sepsis Day 7	whole blood	Sepsis	170243	Sepsis Day 7
GSM812666	sample 58_SIRS Day 0	whole blood	SIRS	169972	SIRS Day 0
GSM812667	sample 59_untreated	whole blood	untreated	169717	untreated
GSM812668	sample 60_SIRS Day 0	whole blood	SIRS	169969	SIRS Day 0
GSM812669	sample 61_SIRS Day 0	whole blood	SIRS	169967	SIRS Day 0
GSM812670	sample 62_untreated	whole blood	untreated	169718	untreated
GSM812671	sample 63_Sepsis Day 0	whole blood	Sepsis	169886	Sepsis Day 0
GSM812672	sample 64_Sepsis Day 7	whole blood	Sepsis	170195	Sepsis Day 7
GSM812673	sample 65_se/ARDS Day 0	whole blood	se/ARDS	169841	se/ARDS Day 0
GSM812674	sample 66_Sepsis Day 0	whole blood	Sepsis	169887	Sepsis Day 0
GSM812675	sample 67_Sepsis Day 7	whole blood	Sepsis	170193	Sepsis Day 7
GSM812676	sample 68_untreated	whole blood	untreated	169721	untreated
GSM812677	sample 69_se/ARDS Day 0	whole blood	se/ARDS	169797	se/ARDS Day 0
GSM812678	sample 70_se/ARDS Day 7	whole blood	se/ARDS	169798	se/ARDS Day 7
GSM812679	sample 71_SIRS Day 0	whole blood	SIRS	169934	SIRS Day 0
GSM812680	sample 72_SIRS Day 0	whole blood	SIRS	169985	SIRS Day 0
GSM812681	sample 73_Sepsis Day 0	whole blood	Sepsis	169888	Sepsis Day 0
GSM812682	sapmle 74_Sepsis Day 7	whole blood	Sepsis	170190	Sepsis Day 7
GSM812683	sample 75_se/ARDS Day 0	whole blood	se/ARDS	169830	se/ARDS Day 0
GSM812684	sample 76_se/ARDS Day 7	whole blood	se/ARDS	169840	se/ARDS Day 7
GSM812685	sample 77_SIRS Day 0	whole blood	SIRS	169956	SIRS Day 0
GSM812686	sample 78_se/ARDS Day 0	whole blood	se/ARDS	169833	se/ARDS Day 0
GSM812687	sample 79_se/ARDS Day 7	whole blood	se/ARDS	169799	se/ARDS Day 7
GSM812688	sample 80_untreated	whole blood	untreated	169723	untreated
GSM812689	sample 81_untreated	whole blood	untreated	169724	untreated
GSM812690	sample 82_untreated	whole blood	untreated	169725	untreated
GSM812691	sample 83_Sepsis Day 0	whole blood	Sepsis	169889	Sepsis Day 0
GSM812692	sample 84_Sepsis Day 7	whole blood	Sepsis	170237	Sepsis Day 7
GSM812693	sample 85_se/ARDS Day 0	whole blood	se/ARDS	169835	se/ARDS Day 0
GSM812694	sample 86_se/ARDS Day 7	whole blood	se/ARDS	169764	se/ARDS Day 7
GSM812695	sample 87_se/ARDS Day 0	whole blood	se/ARDS	169772	se/ARDS Day 0
GSM812696	sample 88_Sepsis Day 0	whole blood	Sepsis	169890	Sepsis Day 0
GSM812697	sample 89_Sepsis Day 7	whole blood	Sepsis	170242	Sepsis Day 7
GSM812698	sample 90_SIRS Day 0	whole blood	SIRS	169971	SIRS Day 0
GSM812699	sample 91_untreated	whole blood	untreated	169727	untreated
GSM812700	sample 92_Sepsis Day 0	whole blood	Sepsis	169891	Sepsis Day 0
GSM812701	sample 93_Sepsis Day 7	whole blood	Sepsis	170198	Sepsis Day 7
GSM812702	sample 94_untreated	whole blood	untreated	169726	untreated
GSM812703	sample 95_Sepsis Day 0	whole blood	Sepsis	169892	Sepsis Day 0
GSM812704	sample 96_Sepsis Day 7	whole blood	Sepsis	170192	Sepsis Day 7
GSM812705	sample 97_se/ARDS Day 0	whole blood	se/ARDS	169770	se/ARDS Day 0
GSM812706	sample 98_se/ARDS Day 7	whole blood	se/ARDS	169765	se/ARDS Day 7
GSM812707	sample 99_se/ARDS Day 0	whole blood	se/ARDS	169728	se/ARDS Day 0
GSM812708	sample 100_se/ARDS Day 7	whole blood	se/ARDS	169771	se/ARDS Day 7
GSM812709	sample 101_SIRS Day 0	whole blood	SIRS	169984	SIRS Day 0
GSM812710	sample 102_Sepsis Day 0	whole blood	Sepsis	169893	Sepsis Day 0
GSM812711	sample 103_Sepsis Day 7	whole blood	Sepsis	170189	Sepsis Day 7
GSM812712	sample 104_Sepsis Day 0	whole blood	Sepsis	170375	Sepsis Day 0
GSM812713	sample 105_Sepsis Day 7	whole blood	Sepsis	170236	Sepsis Day 7
GSM812714	sample 106_Sepsis Day 0	whole blood	Sepsis	170378	Sepsis Day 0
GSM812715	sample 107_Sepsis Day 7	whole blood	Sepsis	170241	Sepsis Day 7
GSM812716	sample 108_Sepsis Day 0	whole blood	Sepsis	170437	Sepsis Day 0
GSM812717	sample 109_Sepsis Day 7	whole blood	Sepsis	170432	Sepsis Day 7
GSM812718	sample 110_Sepsis Day 0	whole blood	Sepsis	170371	Sepsis Day 0
GSM812719	sample 111_Sepsis Day 7	whole blood	Sepsis	170197	Sepsis Day 7
GSM812720	sample 112_SIRS Day 0	whole blood	SIRS	169961	SIRS Day 0
GSM812721	sample 113_se/ARDS Day 0	whole blood	se/ARDS	169768	se/ARDS Day 0
GSM812722	sample 114_SIRS Day 0	whole blood	SIRS	169959	SIRS Day 0
GSM812723	sample 115_SIRS Day 0	whole blood	SIRS	169983	SIRS Day 0
GSM812724	sample 116_Sepsis Day 0	whole blood	Sepsis	170434	Sepsis Day 0
GSM812725	sample 117_Sepsis Day 7	whole blood	Sepsis	170431	Sepsis Day 7
GSM812726	sample 118_Sepsis Day 0	whole blood	Sepsis	170436	Sepsis Day 0
GSM812727	sample 119_Sepsis Day 7	whole blood	Sepsis	170429	Sepsis Day 7
GSM812728	sample 120_untreated	whole blood	untreated	169692	untreated
GSM812729	sample 121_Sepsis Day 0	whole blood	Sepsis	170379	Sepsis Day 0
GSM812730	sample 122_Sepsis Day 7	whole blood	Sepsis	170430	Sepsis Day 7
GSM812731	sample 123_se/ARDS Day 0	whole blood	se/ARDS	169794	se/ARDS Day 0
GSM812732	sample 124_se/ARDS Day 7	whole blood	se/ARDS	170240	se/ARDS Day 7
GSM812733	sample 125_se/ARDS Day 0	whole blood	se/ARDS	170370	se/ARDS Day 0
GSM812734	sample 126_se/ARDS Day 7	whole blood	se/ARDS	170369	se/ARDS Day 7
GSM812735	sample 127_untreated	whole blood	untreated	169691	untreated
GSM812736	sample 128_untreated	whole blood	untreated	169719	untreated
GSM812737	sample 129_se/ARDS Day 0	whole blood	se/ARDS	170374	se/ARDS Day 0
GSM812738	sample 130_se/ARDS Day 7	whole blood	se/ARDS	170377	se/ARDS Day 7
GSM812739	sample 131_se/ARDS Day 0	whole blood	se/ARDS	170376	se/ARDS Day 0
GSM812740	sample 132_se/ARDS Day 7	whole blood	se/ARDS	170372	se/ARDS Day 7
GSM812741	sample 133_untreated_1	whole blood	untreated	128115	untreated
GSM812742	sample 133_untreated_2	whole blood	untreated	128115	untreated
GSM812743	sample 133_untreated_3	whole blood	untreated	128115	untreated
GSM812744	sample 133_untreated_4	whole blood	untreated	128115	untreated
GSM812745	sample 133_untreated_5	whole blood	untreated	128115	untreated
GSM812746	sample 133_untreated_6	whole blood	untreated	128115	untreated
GSM812747	sample 133_untreated_7	whole blood	untreated	128115	untreated
GSM812748	sample 133_untreated_8	whole blood	untreated	128115	untreated
GSM812749	sample 133_untreated_9	whole blood	untreated	128115	untreated
GSM812750	sample 133_untreated_10	whole blood	untreated	128115	untreated
GSM812751	sample 133_untreated_11	whole blood	untreated	128115	untreated
GSM812752	sample 133_untreated_12	whole blood	untreated	128115	untreated

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